Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:233757
Treffy RW, et al., Ephrin-B/EphB Signaling Is Required for Normal Innervation of Lingual Gustatory Papillae. Dev Neurosci. 2016;38(2):124-38
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7834503
|
|
Gene symbol:
|
Efnb2
|
|
Gene name:
|
ephrin B2
|
|
|
Results
|
Specimen
1e/e":
embryonic day 14.5
(more  )
Note: Double labeled: red - Efnb2; green - Uchl1 (assay MGI:7834502).
Specimen
1e/e":
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Efnb2; green - Uchl1 (assay MGI:7834502).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: fungiform papillae |
Present |
Regionally restricted |
1e
,
1e"
|
Intermittent expression along oral surface of lingual epithelium, including fungiform papilla epithelium.
|
| TS22: tongue epithelium |
Present |
Regionally restricted |
1e
,
1e"
|
Intermittent expression along oral surface of lingual epithelium, including fungiform papilla epithelium.
|
Specimen
1f'/f":
embryonic day 15.5
(more )
Note: Double labeled: red - Efnb2; green - Nefm (assay MGI:7834505).
Specimen
1f'/f":
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Efnb2; green - Nefm (assay MGI:7834505).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: fungiform papillae |
Present |
Regionally restricted |
1f'
,
1f"
|
More continuous expression along dorsal lingual epithelium, including fungiform papilla epithelium, not co-localized with Nefm expression.
|
| TS23: tongue epithelium |
Present |
Regionally restricted |
1f'
,
1f"
|
More continuous expression along dorsal lingual epithelium, including fungiform papilla epithelium, not co-localized with Nefm expression.
|
Specimen
1g'/g":
embryonic day 17.5
(more )
Note: Triple labeled: red - Efnb2; green - Gap43 (assay MGI:7834506) and Nefm (assay MGI:7834505).
Specimen
1g'/g":
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - Efnb2; green - Gap43 (assay MGI:7834506) and Nefm (assay MGI:7834505).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: fungiform papillae |
Present |
Regionally restricted |
1g"
,
1g'
|
Expression in serosal surface of fungiform papilla.
|
Specimen
2f:
embryonic day 14.5
(more )
Note: Double labeled: red - Efnb2; green - laminin.
Specimen
2f:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Efnb2; green - laminin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: fungiform papillae |
Present |
Regionally restricted |
2f
|
Expression in afferents just beginning to enter fungiform papilla epithelium.
|
Specimen
2g':
embryonic day 15.5
(more )
Note: Multi labeled: red - Efnb2; blue - Gap43 (assay MGI:7834506) and Nefm (assay MGI:7834505); green - laminin.
Specimen
2g':
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Multi labeled: red - Efnb2; blue - Gap43 (assay MGI:7834506) and Nefm (assay MGI:7834505); green - laminin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tongue epithelium |
Present |
Regionally restricted |
2g'
|
Continuous expression in along dorsal epithelium.
|
|
Analysis Tools