Zap70 MGI Mouse Gene Detail - MGI:99613 - zeta-chain (TCR) associated protein kinase

Symbol

Zap70
Name

zeta-chain (TCR) associated protein kinase
Synonyms

Srk, TZK, ZAP-70

Feature Type

protein coding gene
IDs

MGI:99613
NCBI Gene: 22637
Alliance

gene page
Transcription Start Sites

15 TSS

Sequence Map

Chr1:36800912-36821899 bp, + strand
From NCBI annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 1, 15.41 cM, cytoband A4-C1
Mapping Data

6 experiments

SNPs within 2kb

815 from dbSNP Build 142
Strain Annotations

18
RFLP

1

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_99613	protein coding gene	Chr1:36800879-36821899 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0015932	protein coding gene	Chr1:35322765-35345120 (+)
A/J	MGP_AJ_G0015916	protein coding gene	Chr1:34374511-34393215 (+)
AKR/J	MGP_AKRJ_G0015871	protein coding gene	Chr1:34853921-34873471 (+)
BALB/cJ	MGP_BALBcJ_G0015870	protein coding gene	Chr1:34053791-34072211 (+)
C3H/HeJ	MGP_C3HHeJ_G0015703	protein coding gene	Chr1:35053402-35080277 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0016323	protein coding gene	Chr1:36909783-36936765 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0014045	protein coding gene	Chr1:31412797-31433758 (+)
CAST/EiJ	MGP_CASTEiJ_G0015284	protein coding gene	Chr1:34942303-34964982 (+)
CBA/J	MGP_CBAJ_G0015675	protein coding gene	Chr1:37892737-37920591 (+)
DBA/2J	MGP_DBA2J_G0015776	protein coding gene	Chr1:33702663-33724091 (+)
FVB/NJ	MGP_FVBNJ_G0015778	protein coding gene	Chr1:33226490-33248254 (+)
LP/J	MGP_LPJ_G0015846	protein coding gene	Chr1:35693938-35713942 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0015798	protein coding gene	Chr1:40083949-40110796 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0016370	protein coding gene	Chr1:34901178-34922247 (+)
PWK/PhJ	MGP_PWKPhJ_G0015067	protein coding gene	Chr1:33459462-33482213 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0014849	protein coding gene	Chr1:34842363-34867246 (+)
WSB/EiJ	MGP_WSBEiJ_G0015346	protein coding gene	Chr1:34849588-34874119 (+)

Human Ortholog

ZAP70, zeta chain of T cell receptor associated protein kinase 70

Vertebrate Orthologs

3

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

ZAP70, zeta chain of T cell receptor associated protein kinase 70
Synonyms

ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70
Links

HGNC:12858

NCBI Gene ID: 7535

neXtProt AC: NX_P43403

UniProt: P43403
Chr Location

2q11.2; chr2:97713576-97756364 (+) GRCh38

MGI Vertebrate Homology

Zap70 stringent orthology
1 human;1 mouse;1 rat;1 zebrafish
HCOP

vertebrate homology predictions: ZAP70
Protein SuperFamily

TyrPK_SYK
Gene Tree

Zap70

Diseases

1 with Zap70 mouse models; 2 with human ZAP70 associations

Human Disease

Mouse Models

rheumatoid arthritis

IDs

View 1 model

immunodeficiency 48

IDs

severe combined immunodeficiency

IDs

Click on a disease name to see all genes associated with that disease.

Mutations/Alleles

1 with disease annotations

References

1 with disease annotations

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Phenotype Summary

62 phenotypes from 19 alleles in 23 genetic backgrounds
19 phenotypes from multigenic genotypes
97 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

36
Chemically induced (ENU)

12
Chemically induced (other)

1
Endonuclease-mediated

6
Gene trapped

1
Spontaneous

2
Targeted

12
Transgenic

2
Genomic Mutations

1 involving Zap70
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

55 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Mutant mice show T cell defects. Null mutants lack alpha-beta T cells in the thymus and have fewer T cells in dendritic and intestinal epithelium. Spontaneous and knock-in missense mutations affect T cell receptor signaling, one of the former resulting in severe chronic arthritis.

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All GO Annotations

66
GO References

27

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

741
Tissues

27
cDNA Data

24
Literature Summary

3

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase zap70

ZFIN zap70

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All Sequences

49
RefSeq

10
UniProt

2
CCDS

CCDS14888.1

Ensembl

ENSMUSG00000026117 (Evidence)
NCBI Gene

22637

			Length	Strain/Species	Flank
genomic	22637	NCBI Gene Model \| MGI Sequence Detail	20988	C57BL/6J	± kb
transcript	NM_001289766	RefSeq \| MGI Sequence Detail	2250	ZRU/MplStud
polypeptide	P43404	UniProt \| EBI \| MGI Sequence Detail	618	Not Applicable

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UniProt

2 Sequences
Protein Ontology

PR:000003064 tyrosine-protein kinase ZAP-70

(term hierarchy)
EC

2.7.10.2
InterPro Domains

IPR050198 Non-receptor tyrosine kinases involved in cell signaling

IPR017441 Protein kinase, ATP binding site

IPR000719 Protein kinase domain

IPR011009 Protein kinase-like domain superfamily

IPR001245 Serine-threonine/tyrosine-protein kinase, catalytic domain

IPR000980 SH2 domain

IPR036860 SH2 domain superfamily

IPR035838 SYK/ZAP-70, N-terminal SH2 domain

IPR051184 Tyrosine-phosphorylated adapter molecule

IPR008266 Tyrosine-protein kinase, active site

IPR020635 Tyrosine-protein kinase, catalytic domain

IPR012234 Tyrosine-protein kinase, non-receptor SYK/ZAP-70

IPR023420 Tyrosine-protein kinase SYK/ZAP-70, inter-SH2 domain superfamily

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All nucleic 27

Genomic 1

cDNA 25

Primer pair 1

Microarray probesets 6

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MGD-MRK-16398, MGI:2138217, MGI:3584397, MGI:3584398

Summaries

All 183

Developmental Gene Expression 3

Diseases 1

Gene Ontology 27

Phenotypes 97
Earliest

J:18037 Chan AC, et al., Differential expression of ZAP-70 and Syk protein tyrosine kinases, and the role of this family of protein tyrosine kinases in TCR signaling. J Immunol. 1994 May 15;152(10):4758-66
Latest

J:350708 Eggert J, et al., Cbl-b mitigates the responsiveness of naive CD8(+) T cells that experience extensive tonic T cell receptor signaling. Sci Signal. 2024 Feb 6;17(822):eadh0439

TSS for Zap70:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr6275	Chr1:36800419-36800444 (+)	-480 bp
Tssr6276	Chr1:36800836-36800854 (+)	-67 bp
Tssr6277	Chr1:36800855-36800858 (+)	-55 bp
Tssr6278	Chr1:36800874-36800924 (+)	-13 bp
Tssr6279	Chr1:36800928-36800978 (+)	41 bp
Tssr6280	Chr1:36803593-36803604 (+)	2,687 bp
Tssr6281	Chr1:36806151-36806176 (+)	5,252 bp
Tssr6282	Chr1:36806495-36806506 (+)	5,589 bp
Tssr6283	Chr1:36806540-36806550 (+)	5,633 bp
Tssr6284	Chr1:36809502-36809506 (+)	8,592 bp
Tssr6285	Chr1:36810398-36810448 (+)	9,511 bp
Tssr6286	Chr1:36819027-36819033 (+)	18,118 bp
Tssr42	Chr1:36819101-36819112 (+)	18,195 bp
Tssr6287	Chr1:36819194-36819205 (+)	18,288 bp
Tssr6288	Chr1:36820176-36820193 (+)	19,273 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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