A^iapy
Spontaneous Allele Detail

Summary

• Symbol: A^iapy
• Name: nonagouti; intracisternal A particle yellow
• MGI ID: MGI:1856403
• Synonyms: A*
• Gene: a Location: Chr2:154792519-154892932 bp, + strand Genetic Position: Chr2, 76.83 cM
• Alliance: A^iapy page

Mutation origin

• Strain of Origin: (C57BL/6J x C3H/HeJ)F1/J

Mutation description

• Allele Type: Spontaneous
• Mutation: Transposon insertion
• Mutation details: Southern blot analysis showed that this allele is the result of a 5.2 kb intracisternal A particle (IAP) transposon insertion in an antisense orientation immediately 5' of the first coding exon of the agouti gene. This results in ectopic and differential expression of agouti protein via utilization of a cryptic promoter within the IAP 5' long terminal repeat (LTR) and variable methylation. Methylation assay results indciate that the agouti and yellow mosaic phenotype characteristic of this allele is due to differential epigenetic methylation of the IAP. mRNA from this allele was found to be ectopically over-expressed in several adult tissues from heterozygous mice. The transcript was the same size as mRNA from the wild-type allele. The mutation occurred in a C3H/HeJ A allele. (J:18848, J:21244, J:79708)
• Inheritance: Dominant

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any a Mutation: 463 strains or lines available

Notes

Genbank ID for this allele: L33247, L33248

References

• Original: J:18848 Michaud EJ, et al., Differential expression of a new dominant agouti allele (Aiapy) is correlated with methylation state and is influenced by parental lineage. Genes Dev. 1994 Jun 15;8(12):1463-72
• All: 5 reference(s)