Alpl^tm1Sor
Targeted Allele Detail

Summary

• Symbol: Alpl^tm1Sor
• Name: alkaline phosphatase, liver/bone/kidney; targeted mutation 1, Philippe Soriano
• MGI ID: MGI:1857124
• Synonyms: Tnap^-, TN-AP^Betageo, TNAP-bgeo
• Gene: Alpl Location: Chr4:137469044-137523695 bp, - strand Genetic Position: Chr4, 70.02 cM
• Alliance: Alpl^tm1Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:25249
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette replaced 1.6 kb of the gene, from a portion of exon 2 to a portion of exon 6. The construct deleted the active site of the protein, Ser 93, located in exon 5. RT-PCR of total RNA from E13 gonads of homozygous mutant mice did not detect transcript. In addition, lack of protein in homozygous mutant mice was demonstrated functionally. (J:25249)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Alpl Mutation: 352 strains or lines available

Notes

Only the embryonic (EAP) and tissue non-specific (TNAP) forms of alkaline phosphatase are expressed in mouse embryos. EAP is the predominant form of the enzyme in the early embryo, but predominance switches to TNAP about the 7th embryonic day. Akp2^tm1Sor was used to clarify the expression of TNAP in mouse primordial germ cells. These cells do not express TNAP before gastrulation, but the enzyme is elaborated by extraembryonic tissue prior to that stage. Primordial germ cells do not require TNAP for either development or migration (J:25249).

References

• Original: J:25249 MacGregor GR, et al., Tissue non-specific alkaline phosphatase is expressed in both embryonic and extraembryonic lineages during mouse embryogenesis but is not required for migration of primordial germ cells. Development. 1995 May;121(5):1487-96
• All: 13 reference(s)