Alox5^tm1Fun
Targeted Allele Detail

Summary

• Symbol: Alox5^tm1Fun
• Name: arachidonate 5-lipoxygenase; targeted mutation 1, Colin D Funk
• MGI ID: MGI:1857125
• Synonyms: 5-LO^-, 5LO^-, 5-LO KO, 5-LOX^-, 5-LOX K/O, 5LX-
• Gene: Alox5 Location: Chr6:116387038-116438139 bp, - strand Genetic Position: Chr6, 53.79 cM
• Alliance: Alox5^tm1Fun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:21372
• Parent Cell Line: D3H (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a neomycin resistance cassette into exon 6 disrupted the Alox5 gene. RT-PCR studies did not detect transcript in resident peritoneal macrophages of homozygous mutant mice. Western blot analysis of resident peritoneal macrophages of homozygous mutant mice did not detect ALOX5. (J:21372)

Expression

In Mice Carrying this Mutation:	1 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Alox5 Mutation: 49 strains or lines available

References

• Original: J:21372 Chen XS, et al., Role of leukotrienes revealed by targeted disruption of the 5-lipoxygenase gene. Nature. 1994 Nov 10;372(6502):179-82
• All: 133 reference(s)