Apoe^tm1Unc
Targeted Allele Detail

Summary

• Symbol: Apoe^tm1Unc
• Name: apolipoprotein E; targeted mutation 1, University of North Carolina
• MGI ID: MGI:1857129
• Synonyms: AopE(-), apoE-, apoE0, ApoE-KO, APOE KO, Apoe^tm1Un, EKO, epsilon-, mE^-, mEKO
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^tm1Unc page

Determination of ICAM-1 in aortic roots of tamoxifen treated, high-fat diet fed Apoe^tm1Unc/Apoe^tm1Unc Tg(Cdh5-cre/ERT2)1Rha/0 Nck1^tm1Paw/Nck1^tm1Paw Nck2^tm3Paw/Nck2^tm3Paw mice.

Show the 14 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:1050
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE. (J:1050)

Disease models

Expression

In Mice Carrying this Mutation:	6 assay results 22 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	36 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Apoe^tm1Unc

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 33 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 158 strains or lines available

References

• Original: J:1050 Piedrahita JA, et al., Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4471-5
• All: 2214 reference(s)