Cd247^tm1Lov
Targeted Allele Detail

Summary

• Symbol: Cd247^tm1Lov
• Name: CD247 antigen; targeted mutation 1, Paul E Love
• MGI ID: MGI:1857142
• Synonyms: CD3zeta-KO, zeta -, zeta/eta^-, zeta-KO
• Gene: Cd247 Location: Chr1:165616250-165704846 bp, + strand Genetic Position: Chr1, 73.14 cM, cytoband G-H
• Alliance: Cd247^tm1Lov page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:14170
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Deletion of a genomic fragment containing exon 2 and an insertion of a neomycin gene into exon 4. Northern blot analysis demonstrated that small amounts of a mutant transcript could be detected in thymocyte and lymph node RNA derived from homozygous mice. Metabolic labeling and immunoprecipitation studies also confirmed that the protein was not detectable in thymocytes from homozygous mice. (J:72520)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cd247 Mutation: 11 strains or lines available

References

• Original: J:14170 Love PE, et al., T cell development in mice that lack the zeta chain of the T cell antigen receptor complex. Science. 1993 Aug 13;261(5123):918-21
• All: 30 reference(s)