Csf3^tm1Ard
Targeted Allele Detail

Summary

• Symbol: Csf3^tm1Ard
• Name: colony stimulating factor 3 (granulocyte); targeted mutation 1, Ashley R Dunn
• MGI ID: MGI:1857155
• Synonyms: G-CSF-
• Gene: Csf3 Location: Chr11:98592089-98594455 bp, + strand Genetic Position: Chr11, 62.45 cM
• Alliance: Csf3^tm1Ard page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:20400
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.7kb genomic fragment containing exons 1-3 was replaced by a lacZ gene and neomycin resistance cassette. This mutation placed the lacZ gene under the control of the Csf3 promoter elements. An activity assay demonstrated that the functional protein was not detectable in cultured medium derived from organ cultures of various tissues of homozygous mice. (J:20400)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Csf3 Mutation: 18 strains or lines available

References

• Original: J:20400 Lieschke GJ, et al., Mice lacking granulocyte colony-stimulating factor have chronic neutropenia, granulocyte and macrophage progenitor cell deficiency, and impaired neutrophil mobilization. Blood. 1994 Sep 15;84(6):1737-46
• All: 35 reference(s)