Drd3^tm1Dac
Targeted Allele Detail

Summary

• Symbol: Drd3^tm1Dac
• Name: dopamine receptor D3; targeted mutation 1, Domenico Accili
• MGI ID: MGI:1857159
• Synonyms: D3^-, delta148(+30)
• Gene: Drd3 Location: Chr16:43574389-43643295 bp, + strand Genetic Position: Chr16, 28.44 cM
• Alliance: Drd3^tm1Dac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:32128
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette and sequence encoding 30 non-endogenous residues was inserted into exon 2. RT-PCR analysis on RNA derived from brain of homozygous mice demonstrated the absence of normal transcript. Mutant transcript was detected in both heterozygous and homozygous mutant mice. Binding assays on brain sections derived from homozygous mice confirmed that no functional protein was expressed. Notably, heterozygotes displayed a nearly complete loss of D3-specific binding, to a greater extent than expected for a mutation affecting expression of one allele of the Drd3 gene, raising the possibility that this mutation acts in a dominant-negative fashion. (J:32128)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Drd3 Mutation: 59 strains or lines available

References

• Original: J:32128 Accili D, et al., A targeted mutation of the D3 dopamine receptor gene is associated with hyperactivity in mice. Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1945-9
• All: 55 reference(s)