Kdr^tm1Jrt
Targeted Allele Detail

Summary

• Symbol: Kdr^tm1Jrt
• Name: kinase insert domain protein receptor; targeted mutation 1, Janet Rossant
• MGI ID: MGI:1857168
• Synonyms: flk-1-, Flk1-, flk-1/lacZ, Flk1^lacZ, Flk1lacZ, Flk1-lacZ neo-in, flk.lacZ, VEGFR-2^-, Vegfr2^lacZ
• Gene: Kdr Location: Chr5:76093487-76139118 bp, - strand Genetic Position: Chr5, 40.23 cM
• Alliance: Kdr^tm1Jrt page

Suppression of of extraretinal vascular outgrowth in ischemic Kdr^tm1.1Jamb/Kdr^tm1Jrt Tg(CAG-cre/Esr1*)1Egwa/0 retinas

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:26846
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Insertion of an in-frame lacZ gene and a loxP site flanked neomycin resistance gene cassette replaced the translated portion of the first coding exon and the proximal part of the next intron, and placed lacZ under the transcriptional control of the endogenous promoter. Neither RT-PCR using total RNA nor Western blot analysis using a carboxy terminal antibody detected gene expression in E8.5 homozygous mutant embryos. (J:26846)

Expression

In Mice Carrying this Mutation:	429 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kdr Mutation: 74 strains or lines available

References

• Original: J:26846 Shalaby F, et al., Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature. 1995 Jul 6;376(6535):62-6
• All: 79 reference(s)