Fmr1^tm1Cgr
Targeted Allele Detail

Summary

• Symbol: Fmr1^tm1Cgr
• Name: fragile X messenger ribonucleoprotein 1; targeted mutation 1, Ben Oostra
• MGI ID: MGI:1857169
• Synonyms: Fmr1 KO, Fmr1^tm4Cgr, FMRP KO, fmr-tm1Cgr, FraX
• Gene: Fmr1 Location: ChrX:67722147-67761569 bp, + strand Genetic Position: ChrX, 34.83 cM
• Alliance: Fmr1^tm1Cgr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:19220
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance gene was inserted into exon 5. RT-PCR analysis on testis RNA derived from hemizygous male mice demonstrated that no detectable transcript was produced from this allele, and western blot analysis on extracts of testes, liver, kidney and brain of hemizygous male mice confirmed that no stable encoded protein was made. (J:19220)

Disease models

Expression

In Mice Carrying this Mutation:	112 assay results 9 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Fmr1^tm1Cgr

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any Fmr1 Mutation: 29 strains or lines available

Notes

Genbank: AF179463 and AF170530

References

• Original: J:19220 Bakker CE, et al., Fmr1 knockout mice: a model to study fragile x mental retardation. The Dutch-Belgium Fragile X Consortium. Cell. 1994 Jul 15;78(1):23-33
• All: 596 reference(s)