Gfap^tm1Mes
Targeted Allele Detail

Summary

• Symbol: Gfap^tm1Mes
• Name: glial fibrillary acidic protein; targeted mutation 1, Albee Messing
• MGI ID: MGI:1857176
• Gene: Gfap Location: Chr11:102778162-102791368 bp, - strand Genetic Position: Chr11, 66.48 cM, cytoband D
• Alliance: Gfap^tm1Mes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64290
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette replaced most of exon 1 and all of exons 2-4. Northern blot analysis revealed that there was no mRNA produced from this allele in whole brain RNA samples derived from homozygous mice. Immunohistochemical studies detected no GFAP protein present in sections of hippocampus in homozygous mice. (J:64290)

Expression

In Mice Carrying this Mutation:

13 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gfap Mutation: 48 strains or lines available

References

• Original: J:64290 McCall MA, et al., Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6361-6
• All: 9 reference(s)