Il6^tm1Kopf
Targeted Allele Detail

Summary

• Symbol: Il6^tm1Kopf
• Name: interleukin 6; targeted mutation 1, Manfred Kopf
• MGI ID: MGI:1857197
• Synonyms: Il6-, IL-6-, IL-6 KO, Il6^tm1Koe, Il6^tm1Kopf
• Gene: Il6 Location: Chr5:30218112-30224973 bp, + strand Genetic Position: Chr5, 15.7 cM
• Alliance: Il6^tm1Kopf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:17360
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 2. RT-PCR experiments on RNA derived from LPS-stimulated macrophages of homozygous mice demonstrated that no detectable transcript was produced from this allele. ELISA and bioassay experiments confirmed that no functional protein was made in homozygous mice. (J:17360)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
hm1	Il6^tm1Kopf/Il6^tm1Kopf	B6.129S2-Il6^tm1Kopf/J
hm2	Il6^tm1Kopf/Il6^tm1Kopf	C.129S2(B6)-Il6^tm1Kopf
hm3	Il6^tm1Kopf/Il6^tm1Kopf	involves: 129S2/SvPas
hm4	Il6^tm1Kopf/Il6^tm1Kopf	involves: 129S2/SvPas * C57BL/6
hm5	Il6^tm1Kopf/Il6^tm1Kopf	involves: 129S2/SvPas * C57BL/6J * CE/J
cn6	Il6^tm1Kopf/Il6^+ Prdm1^tm1Clme/Prdm1^tm1Clme Tg(Itgax-cre)1-1Reiz/0	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6 * CBA
cn7	Il6^tm1Kopf/Il6^tm1Kopf Junb^tm3Wag/Junb^tm3Wag Tg(KRT5-cre)1Tak/0	involves: 129/Sv * 129P2/OlaHsd * 129S2/SvPas * C3H * C57BL/6
cx8	Ash1l^Tn(pb-Act-RFP)1.AF0-180T25Zhu/Ash1l^Tn(pb-Act-RFP)1.AF0-180T25Zhu Il6^tm1Kopf/Il6^tm1Kopf	B6.Cg-Ash1l^Tn(pb-Act-RFP)1.AF0-180T25Zhu Il6^tm1Kopf
cx9	Il6^tm1Kopf/Il6^tm1Kopf Tg(ED-L2-IL1RN/IL1B)#Tcw/?	B6.Cg-Il6^tm1Kopf Tg(ED-L2-IL1RN/IL1B)#Tcw
cx10	Il6^tm1Kopf/Il6^+ Tg(ED-L2-IL1RN/IL1B)#Tcw/?	B6.Cg-Il6^tm1Kopf Tg(ED-L2-IL1RN/IL1B)#Tcw
cx11	Il6^tm1Kopf/Il6^+ Ncoa5^tm1Hxia/Ncoa5^+	involves: 129 * 129S2/SvPas * C57BL/6
cx12	Il6^tm1Kopf/Il6^tm1Kopf Inpp5d^tm1Rkh/Inpp5d^tm1Rkh	involves: 129 * C57BL/6
cx13	Il6^tm1Kopf/Il6^tm1Kopf Il6st^tm1Ern/Il6st^tm1Ern	involves: 129S1/Sv * 129S2/SvPas
cx14	Il6^tm1Kopf/Il6^tm1Kopf Lif^tm1Stw/Lif^tm1Stw	involves: 129S1/Sv * 129S2/SvPas * C57BL/6
cx15	Ctsc^tm1Ley/Ctsc^tm1Ley Il6^tm1Kopf/Il6^tm1Kopf	involves: 129S2/SvPas * 129X1/SvJ * C57BL/6
cx16	Il6^tm1Kopf/Il6^tm1Kopf Npc1^m1N/Npc1^m1N	involves: 129S2/SvPas * BALB/c * C57BL/6
cx17	Il6^tm1Kopf/Il6^+ Ncoa5^tm1Hxia/Ncoa5^+	involves: 129S2/SvPas * BALB/c * C57BL/6
cx18	Il6^tm1Kopf/Il6^+ Ncoa5^tm1Hxia/Ncoa5^+	involves: 129S2/SvPas * C57BL/6
cx19	Il6^tm1Kopf/Il6^tm1Kopf Tg(Rorc-EGFP)1Ebe/?	involves: 129S2/SvPas * C57BL/6
cx20	Il6^tm1Kopf/Il6^+ Ncoa5^tm1Hxia/Ncoa5^tm1Hxia	involves: 129S2/SvPas * C57BL/6
cx21	Apc^Min/Apc^+ Il6^tm1Kopf/Il6^tm1Kopf	involves: 129S2/SvPas * C57BL/6
cx22	Foxp3^tm1Kuch/? Il6^tm1Kopf/Il6^tm1Kopf	involves: 129S2/SvPas * C57BL/6
cx23	Il6^tm1Kopf/Il6^tm1Kopf Il6ra^tm1.2Drew/Il6ra^tm1.2Drew	involves: 129S2/SvPas * C57BL/6 * C57BL/6N * FVB/N * SJL
cx24	Il6^tm1Kopf/Il6^tm1Kopf Tg(H2-K-IL6)2Srj/0	involves: 129S2/SvPas * C57BL/6 * DBA/2
cx25	Galc^twi/Galc^twi Il6^tm1Kopf/Il6^tm1Kopf	involves: 129S2/SvPas * C57BL/6J * CE/J
cx26	Il6^tm1Kopf/Il6^tm1Kopf Tank^tm1Aki/Tank^tm1Aki	involves: 129/Sv * 129S2/SvPas * C57BL/6
cx27	Il11ra1^tm1Wehi/Il11ra1^tm1Wehi Il6^tm1Kopf/Il6^tm1Kopf	involves: 129/Sv * C57BL/6

Phenotypes:

Affected Systems	hm1	hm2	hm3	hm4		hm5	cn6	cn7	cx8	cx9	cx10	cx11	cx12	cx13	cx14	cx15	cx16	cx17	cx18	cx19	cx20	cx21	cx22	cx23	cx24	cx25	cx26	cx27
show or hide all annotated terms Sex:
adipose tissue																						N
adipose tissue phenotype																						N
behavior/neurological	√			√	√	√																				√
abnormal learning/memory/conditioning				√	√
abnormal inhibitory learning				√	√
abnormal object recognition memory				√	√
abnormal long-term object recognition memory				√	√
impaired long-term object recognition memory				√	√
abnormal spatial learning				√	√
abnormal emotion/affect behavior	√			√	√
behavioral despair	√			√	√
increased anxiety-related response	√			√	√
increased fear-related response	√
tremors																										√
increased locomotor activity	√
abnormal sleep pattern	√
abnormal frequency of paradoxical sleep	√
seizures						√
cardiovascular system				√	√																					√
abnormal capillary morphology				√	√
abnormal heart morphology				√	√
abnormal heart development				√	√
decreased heart weight				√	√
abnormal heart ventricle morphology				√	√
abnormal interventricular septum morphology				√	√
decreased heart left ventricle wall thickness				√	√
decreased heart left ventricle anterior wall thickness				√	√
decreased heart left ventricle posterior wall thickness				√	√
dilated heart left ventricle				√	√
abnormal cardiovascular system physiology				√	√
decreased cardiac muscle contractility				√	√
abnormal blood-brain barrier function																										√
cellular				√	√							√
teratozoospermia												√
abnormal myoblast migration				√	√
increased sperm motility												√
increased sperm progressive motility												√
abnormal skeletal muscle satellite cell proliferation				√	√
digestive/alimentary system				√	√					√	√																	√
digestive/alimentary phenotype										N	N
increased susceptibility to induced colitis				√	√																							√
esophageal inflammation										√	√
endocrine/exocrine glands	√			√
abnormal pancreatic alpha cell mass	√
abnormal involution of the mammary gland				√
decreased glucagon secretion	√
decreased insulin secretion	√
growth/size/body				√	√
decreased body weight				√	√
obese				√	√
hematopoietic system	√		√	√	√								√							√			√		N
hematopoietic system phenotype																									N
abnormal lymphopoiesis													√
decreased pro-B cell number	√												√
abnormal T cell differentiation				√	√
abnormal CD4-positive T cell differentiation																							√
abnormal myelopoiesis													√
increased regulatory T cell number																							√
decreased T cell number				√	√
abnormal hematopoietic stem cell morphology													√
abnormal effector T cell morphology																				√
abnormal microglial cell morphology				√	√
abnormal T cell physiology			√
decreased cytotoxic T cell cytolysis				√	√
homeostasis/metabolism	√		√	√	√			N							√			N	N				√	√		√
homeostasis/metabolism phenotype								N										N	N
abnormal homeostasis				√	√
decreased circulating interleukin-6 level																							√
increased body temperature	√
abnormal oxygen consumption				√	√
abnormal glucose homeostasis	√
decreased glucagon secretion	√
decreased insulin secretion	√
increased circulating glucose level				√	√
impaired glucose tolerance	√
abnormal acute phase protein level				√	√
abnormal tumor necrosis factor level																										√
abnormal exercise endurance				√	√
decreased incidence of tumors by chemical induction				√	√
abnormal wound healing																								√
abnormal bone healing				√	√
delayed wound healing			√
impaired wound healing			√
decreased susceptibility to injury			√												√
immune system	√		√	√	√		N	√	√	√	√		√	√		N				√			√			√	N	√
immune system phenotype							N	N						N		N											N
abnormal lymphopoiesis													√
decreased pro-B cell number	√												√
abnormal T cell differentiation				√	√
abnormal CD4-positive T cell differentiation																							√
abnormal myelopoiesis													√
increased regulatory T cell number																							√
decreased T cell number				√	√
abnormal effector T cell morphology																				√
abnormal microglial cell morphology				√	√
abnormal T cell physiology			√
decreased cytotoxic T cell cytolysis				√	√
decreased circulating interleukin-6 level																							√
abnormal acute phase protein level				√	√
abnormal tumor necrosis factor level																										√
decreased interferon-gamma secretion	√
decreased interleukin-17 secretion			√																				√
increased interleukin-4 secretion	√
abnormal interleukin-6 secretion	√
decreased susceptibility to experimental autoimmune encephalomyelitis																							√
abnormal inflammatory response			√						√
increased susceptibility to induced colitis				√	√																							√
esophageal inflammation										√	√
decreased susceptibility to induced arthritis	√
increased susceptibility to induced arthritis									√
decreased susceptibility to endotoxin shock														√
skin inflammation								√
increased susceptibility to bacterial infection				√	√
increased susceptibility to bacterial infection induced morbidity/mortality			√
increased susceptibility to Poxviridae infection				√	√
integument				√				√
integument phenotype								N
abnormal involution of the mammary gland				√
skin inflammation								√
hyperkeratosis								√
thick skin								√
liver/biliary system			√	√	√														√
hepatic necrosis				√	√
increased hepatocellular carcinoma incidence																			√
liver degeneration				√	√
abnormal oval cell physiology			√
mortality/aging			√					N													√
mortality/aging								N
increased susceptibility to bacterial infection induced morbidity/mortality			√
preweaning lethality, complete penetrance																					√
muscle				√	√																	N
muscle phenotype																						N
decreased cardiac muscle contractility				√	√
abnormal myoblast migration				√	√
abnormal skeletal muscle satellite cell proliferation				√	√
abnormal skeletal muscle fiber morphology				√	√
neoplasm		√		√	√														√			√
abnormal tumor incidence																						√
decreased incidence of tumors by chemical induction				√	√
increased hepatocellular carcinoma incidence																			√
increased tumor incidence following infection		√
nervous system			√	√	√	√									√		√									√
abnormal cerebellum white matter morphology																										√
abnormal glial cell morphology			√												√
abnormal CNS glial cell morphology				√	√
abnormal microglial cell morphology				√	√
abnormal astrocyte morphology				√	√
gliosis																										√
abnormal neuron morphology				√	√
abnormal brain interneuron morphology				√	√
abnormal axon morphology				√	√
abnormal nervous system physiology				√	√
seizures						√
abnormal blood-brain barrier function																										√
abnormal glial cell physiology																	√
abnormal neuron physiology				√	√
renal/urinary system								N
renal/urinary system phenotype								N
reproductive system												√							N
reproductive system phenotype																			N
teratozoospermia												√
increased sperm motility												√
increased sperm progressive motility												√
enhanced male fertility												√
abnormal epididymis physiology												√
respiratory system				√	√
abnormal pulmonary gas exchange				√	√
skeleton	√			√	√				√
abnormal bone healing				√	√
decreased susceptibility to induced arthritis	√
increased susceptibility to induced arthritis									√
decreased bone mineral density				√	√
vision/eye				√	√
retina outer nuclear layer degeneration				√	√

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Key:

√

disease model

expected model not found

Models:

Human Diseases	cx9	cx24
Barrett's esophagus IDs Barrett's esophagus       DOID:9206 EFO:0000280 ICD10CM:K22.7 ICD9CM:530.85 MESH:D001471 NCI:C2891 OMIM:614266 UMLS_CUI:C0004763 Close
viral infectious disease IDs viral infectious disease       DOID:934 DOID:1329 ICD10CM:A94 ICD10CM:B34.9 ICD9CM:060-066.99 ICD9CM:066.9 MESH:D001102 MESH:D014777 NCI:C3439 NCI:C34396 UMLS_CUI:C0003723 UMLS_CUI:C0042769 Close

Expression

In Mice Carrying this Mutation:	5 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	20 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Il6^tm1Kopf

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 9 strains available Cell Lines: 0 lines available
• Carrying any Il6 Mutation: 38 strains or lines available

References

• Original: J:17360 Kopf M, et al., Impaired immune and acute-phase responses in interleukin-6-deficient mice. Nature. 1994 Mar 24;368(6469):339-42
• All: 712 reference(s)