Myf5^tm1Jae
Targeted Allele Detail

Summary

• Symbol: Myf5^tm1Jae
• Name: myogenic factor 5; targeted mutation 1, Rudolf Jaenisch
• MGI ID: MGI:1857219
• Synonyms: Myf-5 (-), Myf5^Jae, Myf-5^m1
• Gene: Myf5 Location: Chr10:107318769-107321995 bp, - strand Genetic Position: Chr10, 55.95 cM
• Alliance: Myf5^tm1Jae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:3013
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• A PGK-neomycin cassette was inserted into exon 1 of the gene, disrupting the reading frame. (J:3013)

Expression

In Mice Carrying this Mutation:	250 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myf5 Mutation: 17 strains or lines available

Notes

Mice homozygous for Myf6 mutations lack or show reduced Myf5 expression and exhibit phenotypes seen in Myf5^tm1Jae homozygotes. Studies have shown that Myf6 may regulate Myf5 expression by a cis-acting mechanism and that the skeletal abnormalities seen in Myf6 homozygotes may result from a decrease or lack of Myf5 expression (J:24360, J:31636).

References

• Original: J:3013 Braun T, et al., Targeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death. Cell. 1992 Oct 30;71(3):369-82
• All: 38 reference(s)