Myf6^tm1Wb
Targeted Allele Detail

Summary

• Symbol: Myf6^tm1Wb
• Name: myogenic factor 6; targeted mutation 1, Barbara Wold
• MGI ID: MGI:1857220
• Synonyms: MRF4^bh1
• Gene: Myf6 Location: Chr10:107328709-107330584 bp, - strand Genetic Position: Chr10, 55.96 cM
• Alliance: Myf6^tm1Wb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29279
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Replacement of DNA sequences encoding codons 40-173 with a neomycin cassette. This mutation eliminates the helix-loop-helix domain of the protein. Expression of Myf5 is seen at E8, however by E10, it is lower and remains lower. (J:29279)

Expression

In Mice Carrying this Mutation:	118 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myf6 Mutation: 19 strains or lines available

Notes

Studies have shown that Myf6 may regulate Myf5 expression by a cis-acting mechanism and that the skeletal abnormalities seen in Myf6 homozygotes may result from a decrease or lack of Myf5 expression (J:29279, J:42453). This allele (Myf6^tm1Wb) exhibits normal Myf5 expression at E8 but by E10, expression is reduced (J:29279).

References

• Original: J:29279 Patapoutian A, et al., Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome. Development. 1995 Oct;121(10):3347-58
• All: 4 reference(s)