Ncam1^tm1Cgn
Targeted Allele Detail

Summary

• Symbol: Ncam1^tm1Cgn
• Name: neural cell adhesion molecule 1; targeted mutation 1, University of Cologne
• MGI ID: MGI:1857222
• Synonyms: Ncam-, N-CAM-, NCAM^-, N-CAM-1-
• Gene: Ncam1 Location: Chr9:49413436-49710225 bp, - strand Genetic Position: Chr9, 26.83 cM
• Alliance: Ncam1^tm1Cgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:16590
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced most of exons 3 and 4 and the intervening intron. Ribonuclease protection assays did not detect wild-type or read-through transcripts or alternative or cryptic splicing in brain tissue from homozygous mutant mice. Western blot analysis of brain tissue using a monoclonal antibody or a polyclonal rabbit serum did not detect protein in homozygous mutant mice. Similarly, immunostaining did not detect protein in mutant olfactory bulb. (J:16590)

Expression

In Mice Carrying this Mutation:	8 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	10 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Ncam1^tm1Cgn

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ncam1 Mutation: 65 strains or lines available

References

• Original: J:16590 Cremer H, et al., Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning. Nature. 1994 Feb 3;367(6462):455-9
• All: 86 reference(s)