Ngfr^tm1Jae
Targeted Allele Detail

Summary

• Symbol: Ngfr^tm1Jae
• Name: nerve growth factor receptor (TNFR superfamily, member 16); targeted mutation 1, Rudolf Jaenisch
• MGI ID: MGI:1857226
• Synonyms: Ngfr^-, NGFR^tm1Jac, p75-, p75e3-, p75^exonIII-, p75(III)^-, p75-KO, p75^NGFR, p75^NTR-, p75NTR-, p75^NTRexon3-null, p75NTR/ExonIII-, p75^NTR KO
• Gene: Ngfr Location: Chr11:95459644-95478524 bp, - strand Genetic Position: Chr11, 59.01 cM
• Alliance: Ngfr^tm1Jae page

Reduced infarct size in Ngfr^tm1Jae/Ngfr^tm1Jae mice after cardiac ischemia-reperfusion surgery

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43748
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into the third exon of the gene, disrupting the sequences encoding cysteine repeats 2, 3, and 4. Northern blot analysis revealed that the mutant gene did not yield a full length mRNA, however subsequent RT-PCR analysis, described in J:71955, detected an endogenous alternative transcript which lacks exon 3. Western blot analysis showed that the full length isoform was absent in homozygous mutant mice, but an isoform lacking cysteine repeats 2, 3, and 4 was present in both wild and mutant mice. In vitro experiments showed the persisting isoform to be a transmembrane protein that cannot bind neurotrophins but interacts with tyrosine kinase receptors. (J:43748, J:71955)

Expression

In Mice Carrying this Mutation:	34 assay results
In Structures Affected by this Mutation:	22 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ngfr Mutation: 33 strains or lines available

References

• Original: J:43748 Lee KF, et al., Targeted mutation of the gene encoding the low affinity NGF receptor p75 leads to deficits in the peripheral sensory nervous system. Cell. 1992 May 29;69(5):737-49
• All: 205 reference(s)