Nos3^tm1Unc
Targeted Allele Detail

Summary

• Symbol: Nos3^tm1Unc
• Name: nitric oxide synthase 3, endothelial cell; targeted mutation 1, University of North Carolina
• MGI ID: MGI:1857229
• Synonyms: ecNOS-, eNOS-, eNOSKO, NOS3-
• Gene: Nos3 Location: Chr5:24569808-24589472 bp, + strand Genetic Position: Chr5, 11.32 cM
• Alliance: Nos3^tm1Unc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36559
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.2 kb neomycin cassette replaced 129 bp of exon 12 of the gene. This disrupted the calmodulin binding site of the protein and introduced a premature stop codon into the transcripts. Immunohistochemisty of heart and kidney sections from homozygous mutant mice confirmed that no detectable encoded protein was present. (J:36559)

Disease models

Expression

In Mice Carrying this Mutation:	5 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	35 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Nos3 Mutation: 58 strains or lines available

References

• Original: J:36559 Shesely EG, et al., Elevated blood pressures in mice lacking endothelial nitric oxide synthase. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13176-81
• All: 438 reference(s)