Prf1^tm1Sdz
Targeted Allele Detail

Summary

• Symbol: Prf1^tm1Sdz
• Name: perforin 1 (pore forming protein); targeted mutation 1, Sandoz Pharmaceuticals
• MGI ID: MGI:1857235
• Synonyms: P⁰, perf-, perforin-, perforin 0, Pfn^-, pfp-, pfpKO, Pfp^tm1Sdz, pko, Prf^-, Prf1^-, prf1^tm1, Prf1^tm/Sdz
• Gene: Prf1 Location: Chr10:61133612-61140459 bp, + strand Genetic Position: Chr10, 32.18 cM
• Alliance: Prf1^tm1Sdz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:17986
• Parent Cell Line: BL/6-III (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 3. RT-PCR analysis on RNA derived from homozygous mice demonstrated that an abnormal transcript was produced from this allele. However, immunocytochemistry experiments on activated spleen cells derived from homozygous mice confirmed that no detectable protein was made from this allele. (J:17986, J:96542)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 13 strains available Cell Lines: 0 lines available
• Carrying any Prf1 Mutation: 52 strains or lines available

References

• Original: J:17986 Kagi D, et al., Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin-deficient mice [see comments]. Nature. 1994 May 5;369(6475):31-7
• All: 362 reference(s)