Prkcg^tm1Stl
Targeted Allele Detail

Summary

• Symbol: Prkcg^tm1Stl
• Name: protein kinase C, gamma; targeted mutation 1, Susumu Tonegawa
• MGI ID: MGI:1857237
• Synonyms: PKCgamma-KO
• Gene: Prkcg Location: Chr7:3352038-3379615 bp, + strand Genetic Position: Chr7, 1.93 cM
• Alliance: Prkcg^tm1Stl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:16392
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 2.5kb of sequence containing the exon that encodes the nucleotide binding domain. Northern blot analysis of total brain RNA demonstrated that homozygous mutant mice lack normal transcripts. Western blot analysis of total brain protein did not detect the protein in homozygous mutant mice. (J:16392)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Prkcg^tm1Stl/Prkcg^tm1Stl	B6.129-Prkcg^tm1Stl
hm2	Prkcg^tm1Stl/Prkcg^tm1Stl	B6;129P2-Prkcg^tm1Stl/J
hm3	Prkcg^tm1Stl/Prkcg^tm1Stl	involves: 129P2/OlaHsd * BALB/c
hm4	Prkcg^tm1Stl/Prkcg^tm1Stl	involves: 129P2/OlaHsd * C57BL/6
hm5	Prkcg^tm1Stl/Prkcg^tm1Stl	involves: 129S6/SvEvTac * C57BL/6J
hm6	Prkcg^tm1Stl/Prkcg^tm1Stl	involves: 129X1/SvJ * C57BL/6J
hm7	Prkcg^tm1Stl/Prkcg^tm1Stl	Not Specified

Phenotypes:

Affected Systems	hm1	hm2	hm3	hm4	hm5	hm6	hm7
show or hide all annotated terms
behavior/neurological	√		N	√	√	√	√
behavior/neurological phenotype			N	N
alcohol preference					√
enhanced behavioral response to alcohol	√					√
impaired behavioral response to alcohol					√		√
abnormal contextual conditioning behavior				√
abnormal spatial learning				√
decreased anxiety-related response					√
abnormal impulsive behavior control					√
abnormal nociception after inflammation							√
abnormal pain threshold							√
immune system							√
decreased inflammatory response							√
nervous system		√		√
nervous system phenotype				N
abnormal excitatory postsynaptic currents		√
reduced long-term potentiation				√
taste/olfaction					N
taste/olfaction phenotype					N

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkcg Mutation: 42 strains or lines available

References

• Original: J:16392 Abeliovich A, et al., Modified hippocampal long-term potentiation in PKC gamma-mutant mice. Cell. 1993 Dec 31;75(7):1253-62
• All: 42 reference(s)