Prkcg^tm1Stl
Targeted Allele Detail

Summary

• Symbol: Prkcg^tm1Stl
• Name: protein kinase C, gamma; targeted mutation 1, Susumu Tonegawa
• MGI ID: MGI:1857237
• Synonyms: PKCgamma-KO
• Gene: Prkcg Location: Chr7:3352038-3379615 bp, + strand Genetic Position: Chr7, 1.93 cM
• Alliance: Prkcg^tm1Stl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:16392
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 2.5kb of sequence containing the exon that encodes the nucleotide binding domain. Northern blot analysis of total brain RNA demonstrated that homozygous mutant mice lack normal transcripts. Western blot analysis of total brain protein did not detect the protein in homozygous mutant mice. (J:16392)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkcg Mutation: 42 strains or lines available

References

• Original: J:16392 Abeliovich A, et al., Modified hippocampal long-term potentiation in PKC gamma-mutant mice. Cell. 1993 Dec 31;75(7):1253-62
• All: 42 reference(s)