Selp^tm1Hyn
Targeted Allele Detail

Summary

• Symbol: Selp^tm1Hyn
• Name: selectin, platelet; targeted mutation 1, Richard Hynes
• MGI ID: MGI:1857245
• Synonyms: P-, P-selectin^-, P-Selp^-
• Gene: Selp Location: Chr1:163942833-163977595 bp, + strand Genetic Position: Chr1, 71.42 cM
• Alliance: Selp^tm1Hyn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77329
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of exon 3 encoding 10 amino acids of a signal peptide and 27 amino acids of the lectin domain was replaced with a PGK-neo cassette. Mice were treated with lipopolysaccharide to augment the normally low levels of selectin in lung and liver tissue. A longer transcript, resulting from aberrant or cryptic splicing involving the neomycin cassette, was detected at low levels in mutant mice by Northern blot analysis. RT-PCR further confirmed the presence of low levels of transcript in homozygous mice. Immunofluorescence analysis of activated platelets and lung sections from homozygous mutant mice showed an absence of encoded protein. These results were further confirmed by flow cytometric analysis of activated platelets and metabolic labeling of lung tissue. (J:77329)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 12 strains available Cell Lines: 0 lines available
• Carrying any Selp Mutation: 57 strains or lines available

References

• Original: J:77329 Mayadas TN, et al., Leukocyte rolling and extravasation are severely compromised in P selectin-deficient mice. Cell. 1993 Aug 13;74(3):541-54
• All: 79 reference(s)