Spn^tm1Bam
Targeted Allele Detail

Summary

• Symbol: Spn^tm1Bam
• Name: sialophorin; targeted mutation 1, Blair Ardman
• MGI ID: MGI:1857246
• Synonyms: CD43^-
• Gene: Spn Location: Chr7:126731404-126736995 bp, - strand Genetic Position: Chr7, 69.35 cM, cytoband F3-F4
• Alliance: Spn^tm1Bam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29272
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 300 nucleotide fragment encoding a portion of the extracellular domain and all of the transmembrane domain was deleted by via homologous recombination strategies. FACS analysis of splenocytes isolated from homozygous mutant mice showed a lack of the encoded protein on the cell surface. (J:29272)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Spn Mutation: 26 strains or lines available

References

• Original: J:29272 Manjunath N, et al., Negative regulation of T-cell adhesion and activation by CD43. Nature. 1995 Oct 12;377(6549):535-8
• All: 44 reference(s)