Ptprc^tm1Holm
Targeted Allele Detail

Summary

• Symbol: Ptprc^tm1Holm
• Name: protein tyrosine phosphatase receptor type C; targeted mutation 1, Nick Holmes
• MGI ID: MGI:1857277
• Synonyms: CD45^-, CD45-exon9, Ptprc^tm1
• Gene: Ptprc Location: Chr1:137990599-138103446 bp, - strand Genetic Position: Chr1, 60.73 cM
• Alliance: Ptprc^tm1Holm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33466
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette, corrected for a point mutation believed to affect the transformation efficiency of ES cells, was used to disrupt exon 9, which is common to all of the isoforms. The mutation results in the introduction of several stop codons downstream of the insertion. FACS analysis using antibodies for multiple isoforms showed an absence of cell surface expression in homozygous mutant mice. A lack of encoded protein was further confirmed by Western blot analysis of thymus lysates. (J:33466)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 11 strains available Cell Lines: 0 lines available
• Carrying any Ptprc Mutation: 189 strains or lines available

References

• Original: J:33466 Byth KF, et al., CD45-null transgenic mice reveal a positive regulatory role for CD45 in early thymocyte development, in the selection of CD4+CD8+ thymocytes, and B cell maturation. J Exp Med. 1996 Apr 1;183(4):1707-18
• All: 32 reference(s)