Cyp7a1^tm1Rus
Targeted Allele Detail

Summary

• Symbol: Cyp7a1^tm1Rus
• Name: cytochrome P450, family 7, subfamily a, polypeptide 1; targeted mutation 1, David W Russell
• MGI ID: MGI:1857285
• Synonyms: Cyp7-, CYP7A1-
• Gene: Cyp7a1 Location: Chr4:6265612-6275632 bp, - strand Genetic Position: Chr4, 2.91 cM
• Alliance: Cyp7a1^tm1Rus page

Oily coat phenotype of Cyp7a1^tm1Rus/Cyp7a1^tm1Rus nursing mothers and pups

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34342
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing part of exon 3, exon 4 and exon 5 was replaced with a neomycin selection cassette. Activity assays on extracts of liver microsomal memebrane preparations derived from homozygous mice demonstrated that no functional protein is made from this allele. (J:34342)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp7a1 Mutation: 35 strains or lines available

References

• Original: J:34342 Ishibashi S, et al., Disruption of cholesterol 7alpha-hydroxylase gene in mice. I. Postnatal lethality reversed by bile acid and vitamin supplementation. J Biol Chem. 1996 Jul 26;271(30):18017-23
• All: 17 reference(s)