Serpine1^tm1Mlg
Targeted Allele Detail

Summary

• Symbol: Serpine1^tm1Mlg
• Name: serine (or cysteine) peptidase inhibitor, clade E, member 1; targeted mutation 1, Richard C Mulligan
• MGI ID: MGI:1857287
• Synonyms: PAI-1^-, Planh1^tm1, Serpine1^tm1Mg
• Gene: Serpine1 Location: Chr5:137090358-137101122 bp, - strand Genetic Position: Chr5, 76.16 cM
• Alliance: Serpine1^tm1Mlg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39819
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced all of the Serpine1 coding sequence and part of the promoter region including the transcription initiation site. Northern blot analysis did not detect Serpine1 mRNA in endotoxin-treated homozygous mutant liver. Neither untreated nor endotoxin-treated homozygous mutant kidney produced detectable SERPINE1 immunoreactivity. A monoclonal antibody based immunofunctional assay did not detect protein levels in plasma of endotoxin-treated homozygous mutant mice. (J:39819)

Disease models

Expression

In Mice Carrying this Mutation:	29 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Serpine1 Mutation: 25 strains or lines available

References

• Original: J:39819 Carmeliet P, et al., Plasminogen activator inhibitor-1 gene-deficient mice. I. Generation by homologous recombination and characterization. J Clin Invest. 1993 Dec;92(6):2746-55
• All: 166 reference(s)