F2r^tm1Ajc
Targeted Allele Detail

Summary

• Symbol: F2r^tm1Ajc
• Name: coagulation factor II thrombin receptor; targeted mutation 1, Andrew J Connolly
• MGI ID: MGI:1857306
• Synonyms: Par1-, Par1^-, PAR-1^-, tr-
• Gene: F2r Location: Chr13:95738311-95754995 bp, - strand Genetic Position: Chr13, 50.21 cM
• Alliance: F2r^tm1Ajc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33380
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced sequences in exon 2 encoding transmembrane domains 1-7. Northern blot analysis on RNA derived from E12 embryos and from embryonic fibroblasts demonstrated that no detectable transcript was produced from this allele. Furthermore, transcripts from this allele were not detected in various tissues of adult homozygous mice (data not shown). (J:33380)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any F2r Mutation: 26 strains or lines available

References

• Original: J:33380 Connolly AJ, et al., Role of the thrombin receptor in development and evidence for a second receptor. Nature. 1996 Jun 6;381(6582):516-9
• All: 74 reference(s)