Pemt^tm1J
Targeted Allele Detail

Summary

• Symbol: Pemt^tm1J
• Name: phosphatidylethanolamine N-methyltransferase; targeted mutation 1, Jackson
• MGI ID: MGI:1857394
• Synonyms: Pempt2-, Pemt-
• Gene: Pemt Location: Chr11:59861440-59937315 bp, - strand Genetic Position: Chr11, 37.81 cM
• Alliance: Pemt^tm1J page

Small size of Pemt^tm1J/Pemt^tm1J tnyw/tnyw mice (left) with littermate control (right) at 2 weeks of age

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44607
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK neomycin cassette replaces the majority of exon 2, including the translation start site and the extreme 5' end of the intron. Lack of protein in homozygous mutant mice was demonstrated by immunoblot assays on liver homogenates, using a rat antibody from the C terminal end of the protein. Enzymatic activity was also abolished in homozygous mutant animals. (J:44607)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pemt Mutation: 17 strains or lines available

References

• Original: J:44607 Walkey CJ, et al., Disruption of the murine gene encoding phosphatidylethanolamine N-methyltransferase. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):12880-5
• All: 39 reference(s)