Fosb^tm1Meg
Targeted Allele Detail

Summary

• Symbol: Fosb^tm1Meg
• Name: FBJ osteosarcoma oncogene B; targeted mutation 1, Michael E Greenburg
• MGI ID: MGI:1857395
• Synonyms: B^-, FosB-
• Gene: Fosb Location: Chr7:19036621-19043970 bp, - strand Genetic Position: Chr7, 9.56 cM, cytoband A2-B1
• Alliance: Fosb^tm1Meg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34461
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 2, 5' to the sequences encoding the basic region and adjacent to the sequences encoding the transactivation domain. Northern blot analysis on RNA derived from serum-starved, stimulated embryonic fibroblasts derived from homozygous mice demonstrated that no detectable transcript is produced from this allele. Western blot analysis on these same cells confirmed that no detectable protein is encoded by this allele. (J:34461)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fosb Mutation: 22 strains or lines available

References

• Original: J:34461 Brown JR, et al., A defect in nurturing in mice lacking the immediate early gene fosB. Cell. 1996 Jul 26;86(2):297-309
• All: 12 reference(s)