Syk^tm1Paw
Targeted Allele Detail

Summary

• Symbol: Syk^tm1Paw
• Name: spleen tyrosine kinase; targeted mutation 1, Tony Pawson
• MGI ID: MGI:1857421
• Synonyms: Syk^-
• Gene: Syk Location: Chr13:52737209-52802828 bp, + strand Genetic Position: Chr13, 27.41 cM, cytoband B-C2
• Alliance: Syk^tm1Paw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29797
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette replaced an exon that encodes 41 amino acids that are located in the subdomain VI of the kinase domain. Immunoprecipitation and western blot analysis on protein extracts derived from fetal liver showed that no SYK protein was present in homozygous mice. (J:29797)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Syk Mutation: 42 strains or lines available

References

• Original: J:29797 Cheng AM, et al., Syk tyrosine kinase required for mouse viability and B-cell development. Nature. 1995 Nov 16;378(6554):303-6
• All: 10 reference(s)