E2f1^tm1Meg
Targeted Allele Detail

Summary

• Symbol: E2f1^tm1Meg
• Name: E2F transcription factor 1; targeted mutation 1, Michael E Greenburg
• MGI ID: MGI:1857424
• Synonyms: E2f1-, E2F-1^-
• Gene: E2f1 Location: Chr2:154401327-154411812 bp, - strand Genetic Position: Chr2, 76.79 cM
• Alliance: E2f1^tm1Meg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33100
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 3, intron 3 and exon 4 were replaced with a neomycin cassette. These sequences encode residues encoding the leucine zipper and those critical for DNA binding. Northern blot analysis did not detect a transcript made from this allele in fibroblasts isolated from homozygous embryos. (J:33100)

Disease models

Expression

In Mice Carrying this Mutation:	78 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying E2f1^tm1Meg

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any E2f1 Mutation: 28 strains or lines available

References

• Original: J:33100 Field SJ, et al., E2F-1 functions in mice to promote apoptosis and suppress proliferation. Cell. 1996 May 17;85(4):549-61
• All: 98 reference(s)