Alox15^tm1Fun
Targeted Allele Detail

Summary

• Symbol: Alox15^tm1Fun
• Name: arachidonate 15-lipoxygenase; targeted mutation 1, Colin D Funk
• MGI ID: MGI:1857428
• Synonyms: 12/15-LO-, 12/15-LO KO, 12Lox KO, L-12/15-LOX KO, L-12LO-
• Gene: Alox15 Location: Chr11:70234978-70242857 bp, - strand Genetic Position: Chr11, 42.99 cM
• Alliance: Alox15^tm1Fun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44778
• Parent Cell Line: D3H (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a neomycin resistance cassette into exon 3 disrupted the Alox15 gene. Northern blot analyses of resident peritoneal macrophages and of bone marrow-derived macrophages did not detect transcript in homozygous mutant mice. Western blot analysis of resident peritoneal macrophages did not detect ALOX15 in homozygous mutant mice. (J:44778)

Expression

In Mice Carrying this Mutation:	9 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Alox15 Mutation: 45 strains or lines available

References

• Original: J:44778 Sun D, et al., Disruption of 12/15-lipoxygenase expression in peritoneal macrophages. Enhanced utilization of the 5-lipoxygenase pathway and diminished oxidation of low density lipoprotein. J Biol Chem. 1996 Sep 27;271(39):24055-62
• All: 110 reference(s)