Sele^tm1Hyn
Targeted Allele Detail

Summary

• Symbol: Sele^tm1Hyn
• Name: selectin, endothelial cell; targeted mutation 1, Richard Hynes
• MGI ID: MGI:1857450
• Synonyms: E-selectin^-, Sele^-
• Gene: Sele Location: Chr1:163875773-163885246 bp, + strand Genetic Position: Chr1, 71.35 cM
• Alliance: Sele^tm1Hyn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:31626
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A modified D3 ES cell line heterozygous for the Selp^tm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal message for both genes in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in cis with the Selp^tm1Hyn mutation. Due to their genomic proximity, the two null alleles should propagate together through the offspring. (J:31626)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 9 strains available Cell Lines: 0 lines available
• Carrying any Sele Mutation: 51 strains or lines available

References

• Original: J:31626 Frenette PS, et al., Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell. 1996 Feb 23;84(4):563-74
• All: 37 reference(s)