Adra2a^tm1Lel
Targeted Allele Detail

Summary

• Symbol: Adra2a^tm1Lel
• Name: adrenergic receptor, alpha 2a; targeted mutation 1, Lee E Limbird
• MGI ID: MGI:1857459
• Synonyms: alpha2A-AR^D79N, D79N
• Gene: Adra2a Location: Chr19:54033690-54037413 bp, + strand Genetic Position: Chr19, 49.04 cM
• Alliance: Adra2a^tm1Lel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34820
• Parent Cell Line: D3H (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Point mutations were introduced to alter the codon at position 79 from one encoding aspartic acid to one encoding asparagine. RNase protection assays indicated that normal levels of transcript were produced from this allele, but binding assays indicated that no functional protein was present in isolated brain membranes of homozygous mice. (J:34820)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adra2a Mutation: 28 strains or lines available

References

• Original: J:34820 MacMillan LB, et al., Central hypotensive effects of the alpha2a-adrenergic receptor subtype. Science. 1996 Aug 9;273(5276):801-3
• All: 16 reference(s)