Igf1^tm1Ts
Targeted Allele Detail

Summary

• Symbol: Igf1^tm1Ts
• Name: insulin-like growth factor 1; targeted mutation 1, Timothy Stewart
• MGI ID: MGI:1857466
• Synonyms: IGF-I^-
• Gene: Igf1 Location: Chr10:87694127-87772904 bp, + strand Genetic Position: Chr10, 43.7 cM
• Alliance: Igf1^tm1Ts page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:16214
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 3 after codon 15. This mutation inserts multiple stop codons into all reading frames of the B chain of the encoded protein. RT-PCR analysis demonstrated that the transcript is undetectable in homozygous animals. (J:16214)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igf1 Mutation: 29 strains or lines available

References

• Original: J:16214 Powell-Braxton L, et al., IGF-I is required for normal embryonic growth in mice. Genes Dev. 1993 Dec;7(12B):2609-17
• All: 29 reference(s)