Pomc^tm1Low
Targeted Allele Detail

Summary

• Symbol: Pomc^tm1Low
• Name: pro-opiomelanocortin-alpha; targeted mutation 1, Malcolm J Low
• MGI ID: MGI:1857481
• Synonyms: betaend^-, beta-END^-, End-, POMCX*4-
• Gene: Pomc Location: Chr12:4004951-4010642 bp, + strand Genetic Position: Chr12, 1.99 cM
• Alliance: Pomc^tm1Low page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47711
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A PGK-neomycin based targeting vector was used to introduce a premature start codon into exon 3, resulting in the truncation of the translated protein. In situ hybridization on homozygous mutant animals demonstrates that the truncation event had no effect on the distribution or level of transcript. Immunoreactivity demonstrated that Homozygous mutant mice had no detectable beta endorphin activity in the hypothalamus or pituitary (J:78759)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pomc Mutation: 21 strains or lines available

Notes

Additional reference J:65685 identifies the ES cell line origin as 129S2/SvEv. It is more likely that the mice used in this additional reference were derived from the targeted D3 ES cells (129S2/SvPas) first generated in J:78759 and implanted in mice in J:47711.

References

• Original: J:47711 Rubinstein M, et al., Absence of opioid stress-induced analgesia in mice lacking beta-endorphin by site-directed mutagenesis. Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3995-4000
• All: 38 reference(s)