Cyp2e1^tm1Gonz
Targeted Allele Detail

Summary

• Symbol: Cyp2e1^tm1Gonz
• Name: cytochrome P450, family 2, subfamily e, polypeptide 1; targeted mutation 1, Frank J Gonzalez
• MGI ID: MGI:1857483
• Synonyms: Cyp2e1-
• Gene: Cyp2e1 Location: Chr7:140343732-140354903 bp, + strand Genetic Position: Chr7, 85.94 cM
• Alliance: Cyp2e1^tm1Gonz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48196
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was replaced with a neomycin resistance cassette. Northern blot analysis demonstrated that the normal transcript was not present in liver of homozygous mice. Western blot analysis confirmed the absence of the protein in liver extracts derived from homozygous mice. (J:48196)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cyp2e1^tm1Gonz

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp2e1 Mutation: 28 strains or lines available

References

• Original: J:48196 Lee SS, et al., Role of CYP2E1 in the hepatotoxicity of acetaminophen. J Biol Chem. 1996 May 17;271(20):12063-7
• All: 54 reference(s)