Wnt1^tm1Brd
Targeted Allele Detail

Summary

• Symbol: Wnt1^tm1Brd
• Name: wingless-type MMTV integration site family, member 1; targeted mutation 1, Allan Bradley
• MGI ID: MGI:1857490
• Synonyms: Wnt-1-, Wnt1-, Wnt1^neo
• Gene: Wnt1 Location: Chr15:98687738-98691711 bp, + strand Genetic Position: Chr15, 54.65 cM, cytoband F1-F3
• Alliance: Wnt1^tm1Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:10730
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Insertion of a neomycin resistance cassette into the second exon disrupted the Wnt1 gene. (J:10730)

Expression

In Mice Carrying this Mutation:	33 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wnt1 Mutation: 31 strains or lines available

Notes

The phenotype of homozygous mutant mice closely resembles that of Wnt1^sw/Wnt1^sw homozygotes (J:2964). Both are null mutations, and homozygotes for either are characterized by loss of the midbrain and adjacent components of the metencephalon. In null mutation homozygotes, the engrailed genes En1 and En2 are not expressed; this may be the mechanism by which the null mutation affects brain development (J:971). Expression of En-1 in the developing midbrain of Wnt-1-null embryos is sufficient to rescue early midbrain and anterior hindbrain development (J:35491).

References

• Original: J:10730 McMahon AP, et al., The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell. 1990 Sep 21;62(6):1073-85
• All: 25 reference(s)