Crabp2^tm1Ut
Targeted Allele Detail

Summary

• Symbol: Crabp2^tm1Ut
• Name: cellular retinoic acid binding protein II; targeted mutation 1, University of Toronto
• MGI ID: MGI:1857508
• Synonyms: CRABP-II^-
• Gene: Crabp2 Location: Chr3:87856000-87860679 bp, + strand Genetic Position: Chr3, 38.78 cM
• Alliance: Crabp2^tm1Ut page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:23773
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.5 kb genomic fragment containing part of exon 1 including the translation start site was replaced with a neomycin selection cassette. Northern blot analysis on RNA derived from E12.5 homozygous embryos demonstrated that no detectable transcripts were produced from this allele. Immunohistochemistry experiments on sections of spinal cord from homozygous mice confirmed that no detectable protein was made. (J:23773)

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crabp2 Mutation: 30 strains or lines available

References

• Original: J:23773 Fawcett D, et al., Postaxial polydactyly in forelimbs of CRABP-II mutant mice. Development. 1995 Mar;121(3):671-9
• All: 2 reference(s)