Zeb1^tm1Yhi
Targeted Allele Detail

Summary

• Symbol: Zeb1^tm1Yhi
• Name: zinc finger E-box binding homeobox 1; targeted mutation 1, Yujiro Higashi
• MGI ID: MGI:1857515
• Synonyms: deltaEF1^deltaC727
• Gene: Zeb1 Location: Chr18:5591860-5775467 bp, + strand Genetic Position: Chr18, 4.42 cM
• Alliance: Zeb1^tm1Yhi page

Abnormal thymus architecture in Zeb1^tm1Yhi/Zeb1^tm1Yhi mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39865
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The insertion of a neomycin selection cassette into exon 6 introduced a stop codon that precludes translation of the essential zinc finger DNA-binding domain. Northern blot analysis of total embryonic RNA showed the presence of longer transcripts, resulting from the insertion of neo. Western blot analysis using an anti-serum directed against the amino terminus of the encoded protein confirmed the presence of a truncated protein in extracts from homozygous mutant embryos. (J:39865)

Disease models

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Zeb1 Mutation: 65 strains or lines available

References

• Original: J:39865 Higashi Y, et al., Impairment of T cell development in deltaEF1 mutant mice. J Exp Med. 1997 Apr 21;185(8):1467-79
• All: 6 reference(s)