Runx1^tm1Spe
Targeted Allele Detail

Summary

• Symbol: Runx1^tm1Spe
• Name: runt related transcription factor 1; targeted mutation 1, Nancy A Speck
• MGI ID: MGI:1857568
• Synonyms: Cbfa2^-, Cbfa2^rd, Runx1^-, Runx1^deltaE4, Runx1^rd
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm1Spe page

Gross and microscopic views of pathologic lesions in Runx1^tm1Spe/Runx1^tm1Spe mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35115
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4, which encodes a portion of the DNA-binding domain, was replaced with a PGK-neo cassette. RT-PCR analysis showed a lack of normal transcript in homozygous mutant mice. (J:35115)

Expression

In Mice Carrying this Mutation:	13 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:35115 Wang Q, et al., Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3444-9
• All: 25 reference(s)