Braf^tm1Zim
Targeted Allele Detail

Summary

• Symbol: Braf^tm1Zim
• Name: Braf transforming gene; targeted mutation 1, Andreas Zimmer
• MGI ID: MGI:1857579
• Synonyms: B-raf^-, Braf^-, Braf^tm1Pig
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1Zim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41257
• Parent Cell Line: MPI-II (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette was inserted into exon 3, which encodes the N-terminal portion of the Ras-binding domain. Northern blot analysis on RNA derived from E10.5 embryos demonstrated that no detectable transcript is produced from this allele. (J:41257)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 60 strains or lines available

References

• Original: J:41257 Wojnowski L, et al., Endothelial apoptosis in Braf-deficient mice [see comments]. Nat Genet. 1997 Jul;16(3):293-7
• All: 6 reference(s)