Rad51^tm1Hst
Targeted Allele Detail

Summary

• Symbol: Rad51^tm1Hst
• Name: RAD51 recombinase; targeted mutation 1, Paul Hasty
• MGI ID: MGI:1857587
• Synonyms: rad51^m1-
• Gene: Rad51 Location: Chr2:118943295-118966554 bp, + strand Genetic Position: Chr2, 59.92 cM, cytoband F1
• Alliance: Rad51^tm1Hst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36540
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A puromycin selection cassette replaced the region encoding amino acids 73 through 112 and caused a downstream frameshift mutation. RT-PCR analysis of mRNA isolated from homozygous mutant embryos revealed the exclusive presence of a truncated transcript. (J:36540)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad51 Mutation: 31 strains or lines available

References

• Original: J:36540 Lim DS, et al., A mutation in mouse rad51 results in an early embryonic lethal that is suppressed by a mutation in p53. Mol Cell Biol. 1996 Dec;16(12):7133-43
• All: 1 reference(s)