Ptk2^tm1Imeg
Targeted Allele Detail

Summary

• Symbol: Ptk2^tm1Imeg
• Name: PTK2 protein tyrosine kinase 2; targeted mutation 1, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:1857596
• Synonyms: FAK-
• Gene: Ptk2 Location: Chr15:73076951-73295129 bp, - strand Genetic Position: Chr15, 33.94 cM
• Alliance: Ptk2^tm1Imeg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29273
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into sequences encoding the beginning of the kinase domain. Western blot analysis of lysates derived from E8.5 embryos demonstrated that the protein was absent in homozygotes. (J:29273)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Ptk2^tm1Imeg/Ptk2^tm1Imeg	CBA.Cg-Ptk2^tm1Imeg
hm2	Ptk2^tm1Imeg/Ptk2^tm1Imeg	involves: C57BL/6 * CBA
ht3	Ptk2^tm1Imeg/Ptk2^+	CBA.Cg-Ptk2^tm1Imeg

Phenotypes:

Affected Systems	hm1	hm2	ht3
show or hide all annotated terms
cardiovascular system	√	√	√
abnormal blood vessel morphology	√	√
abnormal aorta morphology		√
abnormal vascular development			√
abnormal dorsal aorta morphology		√	√
abnormal vitelline artery morphology		√
abnormal vascular endothelial cell development	√
absent vitelline blood vessels	√
thick myocardium		√
abnormal heart development		√
abnormal mesocardium morphology		√
abnormal endocardium morphology		√
abnormal blood circulation		√
hemorrhage	√	√
cellular		√
abnormal cell morphology		√
abnormal cell migration		√
digestive/alimentary system		√
abnormal foregut morphology		√
embryo	√	√	√
abnormal developmental patterning		√
failure of initiation of embryo turning		√
abnormal ectoderm development		√
abnormal mesoderm development		√
abnormal rostral-caudal axis patterning		√
decreased embryo size		√	√
abnormal embryonic tissue morphology		√
abnormal head fold morphology		√
abnormal head mesenchyme morphology		√
abnormal embryonic neuroepithelium morphology		√
abnormal notochord morphology		√
abnormal somite development		√
abnormal extraembryonic tissue morphology		√
abnormal vitelline artery morphology		√
absent vitelline blood vessels	√
abnormal allantois morphology		√
abnormal amnion morphology		√
small amniotic cavity		√
abnormal visceral yolk sac cavity morphology		√
small visceral yolk sac		√
failure of chorioallantoic fusion		√
growth/size/body		√	√
decreased embryo size		√	√
abnormal head mesenchyme morphology		√
mortality/aging	√	√
embryonic lethality during organogenesis, complete penetrance		√
embryonic lethality between somite formation and embryo turning, complete penetrance	√
muscle		√
thick myocardium		√
nervous system		√
abnormal embryonic neuroepithelium morphology		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	32 assay results
In Structures Affected by this Mutation:	15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptk2 Mutation: 91 strains or lines available

References

• Original: J:29273 Ilic D, et al., Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature. 1995 Oct 12;377(6549):539-44
• All: 14 reference(s)