Nkx2-5^tm1Siz
Targeted Allele Detail

Summary

• Symbol: Nkx2-5^tm1Siz
• Name: NK2 homeobox 5; targeted mutation 1, Seigo Izumo
• MGI ID: MGI:1857618
• Synonyms: Csx/Nkx2.5^-, Nkx2-5^lacZ, Nkx2-5^Lz
• Gene: Nkx2-5 Location: Chr17:27057638-27063962 bp, - strand Genetic Position: Chr17, 13.6 cM
• Alliance: Nkx2-5^tm1Siz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:52597
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• A lacZ-PGK-neomycin resistance cassette replaced all of the coding sequences. (J:52597)

Expression

In Mice Carrying this Mutation:	106 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx2-5 Mutation: 21 strains or lines available

Notes

Homozygous mutant ES cells were injected into C57BL/6 blastocysts to form chimeric animals. Mutant ES cells were shown to contribute to most tissues of surviving animals except heart. Embryos with more than 30-40% contribution of mutant cells to the heart exhibit the phenotype of the homozygous mutant. Embryos with a 20-30% contribution of mutant ES cells to the heart exhibited milder phenotypic defects than the homozygous mutant. Embryos with less than 15% contribution of mutant cells to the heart exhibit no significant heart defects at E10.5(J:52597).

References

• Original: J:52597 Tanaka M, et al., The cardiac homeobox gene Csx/Nkx2.5 lies genetically upstream of multiple genes essential for heart development. Development. 1999 Mar;126(6):1269-80
• All: 28 reference(s)