Rarb^tm1Ipc
Targeted Allele Detail

Summary

• Symbol: Rarb^tm1Ipc
• Name: retinoic acid receptor, beta; targeted mutation 1, Pierre Chambon
• MGI ID: MGI:1857623
• Synonyms: Abeta, Rarb2-, RARbeta2^-
• Gene: Rarb Location: Chr14:5650540-6038924 bp, + strand Genetic Position: Chr14, 7.08 cM, cytoband A1-A3
• Alliance: Rarb^tm1Ipc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:21486
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Ablation of the expression of the most abundant isoform, Rarbeta2, encoded by this locus was achieved in two steps. First, all of the in-frame start codons for the Rarbeta2 isoform were eliminated by site-specific mutagenesis of nucleotides 459 through 491. Second, a neomycin resistance gene was inserted into the first exon of the Rarbeta2 isoform, exon 4. RT-PCR and Western blot analysis, using isoform specific primers and antibodies, showed a lack of Rarbeta2 mRNA and protein, as well as normal levels of transcript and protein corresponding to the other isoforms. (J:21486)

Expression

In Mice Carrying this Mutation:

70 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rarb Mutation: 40 strains or lines available

References

• Original: J:21486 Mendelsohn C, et al., Retinoic acid receptor beta 2 (RAR beta 2) null mutant mice appear normal. Dev Biol. 1994 Nov;166(1):246-58
• All: 15 reference(s)