Pcgf2^tm1Hko
Targeted Allele Detail

Summary

• Symbol: Pcgf2^tm1Hko
• Name: polycomb group ring finger 2; targeted mutation 1, Haruhiko Koseki
• MGI ID: MGI:1857633
• Synonyms: mel-18-, mel18^-
• Gene: Pcgf2 Location: Chr11:97579649-97591323 bp, - strand Genetic Position: Chr11, 61.06 cM, cytoband C1-C3
• Alliance: Pcgf2^tm1Hko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33159
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exon 5, which contains the transcriptional initiation codon and encodes the RING finger domain. RNAse protection assays demonstrated that a truncated transcript is produced from this allele in homozygous mice, but the authors predict that no functional protein can be translated. (J:33159)

Expression

In Mice Carrying this Mutation:	342 assay results
In Structures Affected by this Mutation:	12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pcgf2 Mutation: 48 strains or lines available

References

• Original: J:33159 Akasaka T, et al., A role for mel-18, a Polycomb group-related vertebrate gene, during the anteroposterior specification of the axial skeleton. Development. 1996 MAY;122(5):1513-1522
• All: 19 reference(s)