Edn1^tm1Utj
Targeted Allele Detail

Summary

• Symbol: Edn1^tm1Utj
• Name: endothelin 1; targeted mutation 1, University of Tokyo
• MGI ID: MGI:1857637
• Synonyms: EdnRA^-, ET-1^-
• Gene: Edn1 Location: Chr13:42454952-42461466 bp, + strand Genetic Position: Chr13, 20.82 cM
• Alliance: Edn1^tm1Utj page

Cardiovascular abnormalities in Edn1^tm1Utj/Edn1^tm1Utj mice at 18.5 dpc

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:17694
• Parent Cell Line: A3-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance gene was inserted into exon 2, which encodes the mature peptide. RT-PCR analysis on RNA derived from homozygous embryos demonstrated that no detectable transcript was produced from this allele. Enzyme immunoassay tests confirmed that no active peptide was present in lung extracts of homozygous mice. (J:17694)

Expression

In Mice Carrying this Mutation:	65 assay results
In Structures Affected by this Mutation:	25 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Edn1 Mutation: 26 strains or lines available

References

• Original: J:17694 Kurihara Y, et al., Elevated blood pressure and craniofacial abnormalities in mice deficient in endothelin-1 [see comments]. Nature. 1994 Apr 21;368(6473):703-10
• All: 31 reference(s)