Rac2^tm1Mddw
Targeted Allele Detail

Summary

• Symbol: Rac2^tm1Mddw
• Name: Rac family small GTPase 2; targeted mutation 1, Mary C Dinauer and David A Williams
• MGI ID: MGI:1857701
• Synonyms: rac2-, Rac2^tm1Daw
• Gene: Rac2 Location: Chr15:78443369-78456983 bp, - strand Genetic Position: Chr15, 37.57 cM
• Alliance: Rac2^tm1Mddw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53362
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing exon 1, which encodes the translational initiation site and part of the GTP-binding domain. Northern blot and RT-PCR analysis demonstrated that no transcripts were detectable in total RNA derived from neutrophils or lymphocytes in homozygous mice. Western blot analysis confirmed an absence of protein produced from this allele in peritoneal exudate cells from homozygous mice. (J:53362)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rac2 Mutation: 17 strains or lines available

References

• Original: J:53362 Roberts AW, et al., Deficiency of the hematopoietic cell-specific Rho family GTPase Rac2 is characterized by abnormalities in neutrophil function and host defense. Immunity. 1999 Feb;10(2):183-96
• All: 76 reference(s)