Jag1^tm1Grid
Targeted Allele Detail

Summary

• Symbol: Jag1^tm1Grid
• Name: jagged 1; targeted mutation 1, Tom Gridley
• MGI ID: MGI:1857713
• Synonyms: Jag1^dDSL, Jag1^del1, Jag1^null
• Gene: Jag1 Location: Chr2:136923371-136958440 bp, - strand
• Alliance: Jag1^tm1Grid page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54907
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 5kb genomic fragment at the 5' end of the gene was replaced by a neomycin resistance cassette. These deleted sequences include coding regions for the C-terminal portion of the DSL domain. RT-PCR analysis demonstrated that no transcript was detectable in RNA isolated from E10 homozygous embryos, and Western blot analysis on extracts of E10.5 homozygous embryos confirmed that the protein was not expressed. (J:54907)

Disease models

Expression

In Mice Carrying this Mutation:	36 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	18 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Jag1 Mutation: 78 strains or lines available

References

• Original: J:54907 Xue Y, et al., Embryonic lethality and vascular defects in mice lacking the Notch ligand Jagged1. Hum Mol Genet. 1999 May;8(5):723-30
• All: 30 reference(s)