Vcl^tm1Eda
Targeted Allele Detail

Summary

• Symbol: Vcl^tm1Eda
• Name: vinculin; targeted mutation 1, Eileen D Adamson
• MGI ID: MGI:1857730
• Synonyms: Vcl⁰, vinculin^-
• Gene: Vcl Location: Chr14:20979466-21083744 bp, + strand Genetic Position: Chr14, 11.53 cM
• Alliance: Vcl^tm1Eda page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45781
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon E3 was replaced by a neomycin cassette. Homozygous mutant embryos do not express vinculin protein, as assesed by Western blotting. The strategy employed to inactivate the vinculin gene leads to impaired production of vinculin as well as metavinculin. Heterozygotes exhibit a 58% reduction of vinculin and a 63% reduction of its muscle splice variant (metavinculin) protein levels versus wild-type littermates. (J:45781, J:92398)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	21 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vcl Mutation: 48 strains or lines available

References

• Original: J:45781 Xu W, et al., Vinculin knockout results in heart and brain defects during embryonic development. Development. 1998 Jan;125(2):327-37
• All: 4 reference(s)